Solution structure of a PAN module from the apicomplexan parasite Eimeria tenella |
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Authors: | Brown Philip J. Mulvey Denise Potts Jennifer R. Tomley Fiona M. Campbell Iain D. |
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Affiliation: | (1) Institute for Animal Health, Compton, Newbury, Berkshire, RG20 7NN, UK;(2) Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK |
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Abstract: | Micronemes, specialised organelles found in all apicomplexan parasites, secrete molecules that are essential for parasite attachment and invasion of host cells. EtMIC5 is one such microneme protein that contains eleven tandemly repeating modules. These modules have homology with the PAN module superfamily. Members of this family are found in blood clotting proteins, some growth factors and some nematode proteins. This paper presents the structure of the 9th PAN module in EtMIC5, determined using high resolution NMR. The structure shows similarities to and some differences from the N-terminal module of hepatocyte growth factor (HGF), the only previous member of the PAN family with known structure. AbbreviationsNMR – nuclear magnetic resonance; NOE – nuclear Overhauser enhancement; NOESY – NOE spectroscopy; COSY – correlated spectroscopy; TOCSY – total correlated spectroscopy; HSQC – hetero nuclear single quantum coherence; HMQC-J – hetero nuclear multiple quantum coherence-J coupling; MICs – microneme proteins; EtMIC5 – a microneme protein from Eimeria tenella; Apple9 – the ninth Apple repeat of EtMIC5; FXI – blood coagulation factor XI; PK – plasma prekallikrein; HGF – hepatocyte growth factor. |
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Keywords: | Apple Eimeria tenella NMR PAN module |
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