Biochemical changes accompanying brefeldin a formation inCurvularia lunata |
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Authors: | C T Sam |
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Institution: | (1) Department of Pharmacy, University of Sydney, 2006 Sydney, New South Wales, Australia;(2) Present address: 35, Pasir Ris Terrace, Singapore 18, Republic of Singapore |
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Abstract: | Extracellular brefeldin A was detected in 4 % glucose-peptone-mineral salts cultures ofCurvularia lunata at the start of the exponential growth phase. Some fluctuations in brefeldin A levels occurred during the exponential growth
phase followed by a significant reduction in level at the stationary growth phase. Broth glucose levels decreased according
to a sigmoid relationship with time whereas broth pH remained fairly constant during the exponential growth phase followed
by a gradual increase into the stationary growth phase. Mycelial brefeldin A levels were low throughout the various growth
phases. The principal fatty acids present in decreasing order during the exponential growth phase were linoleic, oleic and
palmitic acids. However, the content of linoleic acid was significantly reduced at the onset and during the stationary growth
phase. |
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Keywords: | |
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