Cadmium induces a novel metallothionein and phytochelatin 2 in an aquatic fungus |
| |
Authors: | Jaeckel Petra Krauss Gudrun Menge Sieglinde Schierhorn Angelika Rücknagel Peter Krauss Gerd-Joachim |
| |
Affiliation: | UFZ Centre for Environmental Research Leipzig-Halle in the Helmholtz Association, Department of Environmental Microbiology, Halle/Saale, Germany. |
| |
Abstract: | Cadmium stress response was measured at the thiol peptide level in an aquatic hyphomycete (Heliscus lugdunensis). In liquid culture, 0.1 mM cadmium increased the glutathione (GSH) content and induced the synthesis of additional thiol peptides. HPLC, electrospray ionization mass spectrometry, and Edman degradation confirmed that a novel small metallothionein as well as phytochelatin (PC2) were synthesized. The metallothionein has a high homology to family 8 metallothioneins (http://www.expasy.ch/cgi-bin/lists?metallo.txt). The bonding of at least two cadmium ions to the metallothionein was demonstrated by mass spectrometry (MALDI MS). This is the first time that simultaneous induction of metallothionein and phytochelatin accompanied by an increase in GSH level has been shown in a fungus under cadmium stress, indicating a potential function of these complexing agents for in vivo heavy metal detoxification. The method presented here should be applicable as biomarker tool. |
| |
Keywords: | Cadmium Phytochelatin Metallothionein Mass spectrometry ESI MALDI-PSD Fungi Aquatic hyphomycetes Heliscus lugdunensis |
本文献已被 ScienceDirect PubMed 等数据库收录! |