Expression of heat shock protein in host macrophages correlates with a protective potential against infection with Leishmania major in mice |
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Authors: | Hiroyuki Ishikawa Hajime Hisaeda Yoichi Maekawa Hideyuki Nagasawa Tohru Sakai Fusao Ota Kunisuke Himeno |
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Institution: | aDepartment of Parasitology and Immunology, University of Tokushima School of Medicine, Kuramoto 3, Tokushima 770, Japan bDepartment of Food Microbiology, University of Tokushima School of Medicine, Tokushima, Japan |
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Abstract: | We studied the relationship between the expression of 65-kDa heat shock protein (HSP65) and resistance of mice to infection with Leishmania major (L. major), an obligate intracellular protozoan. C57BL/6 (B6) mice, a strain genetically resistant to L. major infection, expressed high level of HSP65 in their peritoneal and draining lymph node macrophages after infection, whereas susceptible BALB/c mice expressed only slightly at the early stage of infection. This protein was not expressed in the parasite itself and macrophages of non-infected mice. We examined the role of T cells in the expression of HSP65 by using SCID mice grafted with the fetal thymus from B6 or BALB/c mice (B6-TG or BALB-TG mice, respectively). Either BALB-TG or B6-TG mice were reconstituted with T cells derived from each grafted fetal thymus. B6-TG mice were markedly resistant against infection with L. major, compared with BALB-TG mice. Furthermore, the HSP65 expression in macrophages of thymus-grafted mice was similar to that of the thymus-donor type. That is, B6-TG mice expressed a high level of this protein, whereas BALB-TG mice did in lower level than B6-TG mice. These results show that T cells are necessary to express HSP65 and the expression correlates with a protective potential of T cells against infection with L. major. |
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Keywords: | Heat shock protein Leishmania major SCID mice Protection Immunity to infection |
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