首页 | 本学科首页   官方微博 | 高级检索  
     


A simple method for recovering DNA from agarose gels using glass fibre filters
Authors:Vijeshwar Verma  Gulam N. Qazi
Affiliation:(1) Genetic Engineering Unit, Regional Research Laboratory, Canal Road, 180 001 Jammu Tawi, India
Abstract:A simple and reproducible procedure for the recovery of plasmid DNA is described. The method was standardised for the purification of plasmids from Gluconobacter oxydans ATCC9937. The protocol is based on the use of glass microfibre filter paper for entrapment of DNA and its subsequent recovery by an elution buffer. The method precludes the use of phenol and butanol for the removal of proteins and ethidium bromide respectively, therefore, making the procedure inexpensive and gentle.
Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号