Cytochalasin B-induced alterations of insulin binding and microfilament organization in cultured fibroblasts |
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Authors: | Mohan K Raizada Robert E Fellows B Wu |
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Institution: | 1. Department of Physiology, University of Florida, College of Medicine, Gainesville, FL 32160 USA;2. Department of Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, USA |
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Abstract: | The effect of cytochalasin B (CB) on insulin binding has been investigated in confluent cultures of chick embryo fibroblasts. Time- and dose-dependent increases in binding of 125I]insulin was observed after incubation of fibroblasts with CB. At 10 μg/ml, CB caused a 2-fold increase in binding, due to an increase in the number of binding sites from 9.3 × 103 to 2.0 × 104 per cell. Removal of CB from the growth medium was accompanied by a decrease in 125I]insulin binding to control values in 24 h. Increase in the binding of insulin in CB-treated CEF was also accompanied by enhancement of insulin to stimulation of 3H]thymidine incorporation into acid-insoluble material. CB treatment also caused disorganization and disappearance of microfilament bundles and changes in cell shape from flat, with a few blebs and folds on the cell surface, to round with numerous blebs and folds. The data from this study suggest that changes in the number of surface insulin-binding sites may be related to the state of organization of cytoskeletal structures in chick embryo fibroblasts. |
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Keywords: | To whom offprint requests should be addressed |
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