L3 and adult Ostertagia (Teladorsagia) circumcincta exhibit cyanide sensitive oxygen uptake |
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| Authors: | Simcock D C Brown S Neale J D Przemeck S M C Simpson H V |
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| Institution: | Laboratory for Biochemical Parasitology, Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand. D.C.Simcock@massey.ac.nz |
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| Abstract: | Oxygen consumption by L3 and adult Ostertagia (Teladorsagia) circumcincta was examined in vitro to determine whether oxygen can be utilised in metabolism. The oxygen concentration in the abomasal fluid of sheep infected with O. circumcincta was also measured. Rates of consumption (in nmol O2/h/1000 worms) were 13+/-1 in sheathed L3, 34+/-6 in ex-sheathed L3, and 1944+/-495 in adult worms. Constant rates of consumption were maintained until media oxygen concentration dropped to between 10 and 20 microM. Consumption was inhibited 95% by cyanide in L3 and 74% in adults. Oxygen concentration in abomasal fluid varied between 10 and 30 microM in both infected and uninfected animals. During infection, oxygen concentration decreased slightly with increased abomasal pH, though the correlation between the two was poor (r=-0.30). In conclusion, O. circumcincta can consume oxygen and oxygen concentration at the infection site is sufficient to support at least some aerobic metabolism. |
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| Keywords: | Ostertagia circumcincta Teladorsagia circumcincta Nematode Oxygen consumption Abomasum Aerobic metabolism Anaerobic metabolism ATP adenosine triphosphate NAD+ nicotinamide adenine dinucleotide NADH nicotinamide adenine dinucleotide hydrogenated Hepes N-2-hydroxyethylpiperazine-N′-2-ethanesulphonic acid mmHg millimeters of mercury g gravity p i post-infection v/v volume to volume O2 oxygen L2 second stage larvae L3 third stage larvae L4 fourth stage larvae Pa Pascals NaOCl sodium hypochlorite KH2PO4 potassium phosphate KCN potassium cyanide mOsm milliosmoles min minutes h hours s seconds ROS reactive oxygen species |
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