Biochemical and Immunochemical Investigations on the Light-Harvesting System of the Cryptophyte Rhodomonas sp.: Evidence for a Photosystem I Specific Antenna |
| |
Authors: | L. Bathke E. Rhiel W. E. Krumbein J. Marquardt |
| |
Affiliation: | ICBM/Geomikrobiologie, Carl von Ossietzky-Universität Oldenburg, Oldenburg, Germany |
| |
Abstract: | Abstract: Thylakoid membranes of the cryptophyte Rhodomonas sp. were solubilized with the mild detergent dodecyl-β-maltoside and subjected to sucrose density gradient centrifugation. The resulting gradients showed six pigment-bearing bands which were characterized further by means of absorption and fluorescence emission (77K) spectroscopy, polyacrylamide gel electrophoresis and Western immunoblotting. Two of the bands showed characteristics of light-harvesting complexes, other bands could be attributed to photosystem II and photosystem I. Up to 10 different light-harvesting proteins could be identified, some of which are specific for photosystem I, others for photosystem II. The polypeptides of the light-harvesting complex of photosystem II show a higher chlorophyll c/a ratio than the antenna proteins of photosystem I. As in vascular plants, they represent the bulk of the membrane-intrinsic light-harvesting proteins. |
| |
Keywords: | Rhodomonas Cryptophyceae photosynthesis light-harvesting complexes photosystems |
|
|