杉木再生系统的比较研究

通过对杉木(Cunninghamia lanceolata Hook)再生系统的比较研究,建立了子叶、下胚轴、茎段和针叶等四个再生体系。子叶在附含1mg/L BA和0.1mg/L NAA的DCR培养基中再生频率为93.7%±0.45%,平均芽数为3.76±0.25;下胚轴在附含2mg/L BA和0.2mg/L NAA的DCR培养基中再生频率为96.2%±0.35%,平均芽数为17.4±0.18;茎段在附含1mg/L BA和0.1mg/L NAA的DCR培养基中再生频率达100%,平均芽数为3.28±0.11;针叶在附含1.5mg/L BA和0.1mg/L NAA的DCR培养基中再生频率达84.5%±0.45%,平均芽数仅为1.42±0.08。不定芽在附含0.2mg/L BA和0.02mg/L NAA的DCR培养基中有效伸长。芽苗经预处理后在附含0.3mg/L IBA的1/2 DCR培养基中生根效果最佳。

COMPARATIVE STUDY ON REGENERATION SYSTEMS OF CUNNINGHAMIA LANCEOLATA HOOK

Four regeneration systems derived from cotyledon, hypocotyls, stem and needle explants have been established in the comparative researches of regeneration systems in Cunninghamia lanceolata Hook. A high frequency (93.7% +/- 0.45%) of adventitious buds were induced from cotyledons on DCR medium supplemented with 1 mg/L BA and 0.1 mg/L NAA, with a maximum mean number buds of 3.76 +/- 0.25. A higher frequency (96.2% +/- 0.35%) of adventitious buds were induced from hypocotyls on DCR medium supplemented with 2 mg/L BA and 0.2 mg/L NAA, with a maximum mean number buds of 17.4 +/- 0.18. A highest frequency (100%) of adventitious buds were induced from stem segments on DCR medium supplemented with 1 mg/L BA and 0.1 mg/L NAA, with a maximum mean number buds of 3.28 +/- 0.11. A low frequency (84.5% +/- 0.45%) of adventitious buds were induced from needles on DCR medium supplemented with 1.5 mg/L BA and 0.1 mg/L NAA, with a maximum mean number buds of 1.42 +/- 0.08. Buds were elongated on DCR medium supplemented with 0.2 mg/L BA and 0.02 mg/L NAA. After pre-treating shoots, best rooting result was produced on 1/2 DCR medium supplemented with 0.3 mg/L IBA.