Variability for resistance to Fusarium solani culture filtrate and fusaric acid among somaclones in pea |
| |
| Authors: | J Horáček L Švábová P Šarhanová A Lebeda |
| |
| Institution: | 1131. Biotechnology Department, Agritec Plant Research Ltd., Zemědělská 16, CZ-78701, ?umperk, Czech Republic
2131. Faculty of Science, Department of Botany, Palacky University in Olomouc, ?lechtitel? 11, CZ-78371, Olomouc-Holice, Czech Republic
|
| |
| Abstract: | Pea (Pisum sativum L.) somaclones of cultivars Adept, Komet and Bohatýr were obtained after selection in vitro with Fusarium solani filtrate and fusaric acid (FA). R2 regenerants were analysed by random amplification of polymorphic DNA (RAPD; OPAB4, P-14, UBC-556) and inter-retrotransposon amplification polymorphism (IRAP; Ogre) markers. Marker UBC-556 showed different banding patterns for each cultivar, but without specific bands for selected and control plants. Markers OPAB4, P14 and Ogre were useful for clear discrimination between selected and non-selected variants of all three cultivars. Flow cytometry analysis proved the same genome size of selected and non-selected pea lines. Therefore in vitro selection by pathogen derived agents could be the efficient method for obtaining of pea somaclones with increased resistance to F. solani. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
