马尾松叶生化物质的生物检测与物质鉴定

马尾松是我国特有的乡土树种,长期林业生产实践表明马尾松纯林连栽会引起马尾松生产量下降。本实验采用超临界萃取技术提取马尾松叶生化物质,并采用马尾松种子发芽试验进行生物检测及物质鉴定。生物检测结果表明,纯CO2提取的马尾松叶生化物质以及乙醇和CO2混和提取的马尾松叶生化物质对马尾松种子的发芽具有不同的效应。总体效果而言,纯CO2提取的马尾松叶生化物质对马尾松种子发芽率、胚根长、鲜重和干重具有不同程度的抑制效应,而对发芽势及胚轴长则具有促进效应;乙醇和CO2混和提取的马尾松叶生化物质对马尾松种子发芽率、胚轴长、鲜重和干重具有不同程度的抑制作用,而对发芽势及胚根长则具有促进作用。物质鉴定结果表明纯CO2提取的马尾松叶生化物质主要是苯甲醛、Cis-2,6-二甲基-2,6-辛二烯等,乙醇和CO2混和提取的马尾松叶生化物质主要是β-芳樟醇、苯乙醇等。

Bio-detection and Identification of Biochemical Substances in Pinus massoniana Leaves

Pinus massoniana is a specific landrace species and long-term production practices of forestry indicate that the continuous cropping of pure P. massoniana forests can result in decreased P. massoniana yields. This study adopted Supercritical Fluid Extraction to extract biochemical substances from P. massoniana leaves and germination test to biologically detect and identify these substances. The results of the bio-detection indicated that the substances extracted only with CO2 and the substances extracted with the mixture of CO2 and ethanol showed different effects on the germination of P. massoniana seeds. As a whole, the substances extracted only with only CO2 appeared to inhibit the germination rate and radicle length and fresh and dry weight of P. massoniana seeds to a varying extent but to increase the germination potential and hypocotyl length of the seeds and the substances extracted with the mixture of CO2 and ethanol appeared to inhibit the germination rate and hypocotyl length and fresh and dry weights of P. massoniana seeds but to increase the germination potential and radicle length of the seeds. The results of the identification revealed that of the substances extracted only with CO2,the major ones were benzaldehyde and cis-2,6-Dimethyl-2,6-octadiene and of the substances extracted with the mixture of CO2 and ethanol,the major ones were β-linalool and phenethyl alcohol.