Thidiazuron-induced morphogenesis in tamarind seedlings |
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Authors: | Urmil?J?Mehta N?Sahasrabudhe Email author" target="_blank">Sulekha?HazraEmail author |
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Institution: | (1) Plant Tissue Culture Division, National Chemical Laboratory, 411008 Pune, India |
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Abstract: | Summary Germination of tamarind seeds in medium containing thidiazuron (TDZ) resulted in induction of nodular protrusions in and around
the cotyledonary node meristem. The structures developed radially in well-defined circles and subsequently spread towards
the cotyledonary bridge and also in the proximal part of the hypocotyl. The structures developed into shoots on transfer to
medium devoid of growth regulators. Histological studies revealed that the protrusions initiated from the nodal meristem and
extended to the non-meristematic region between the two meristems and also in the proximal part of the hypocotyl in seedlings
germinated in 9.08 μM TDZ. Newly formed cell layers and less-differentiated meristematic protrusions were also seen. With the increase in the distance
from the meristem, the buds were less differentiated; in the proximal part of the hypocotyl only the multiple layers of meristematic
cells were noted. With extension of the period of incubation, the TDZ-induced meristematic activity extended laterally in
circles towards the neighboring region. The radial spread of the meristematic activity from the center of the nodal meristem
was also evident at 18.16 μM TDZ. From the pattern of the morphogenic development and the histological studies it may be hypothesized that in tamarind,
TDZ influences the existing meristems specifically. Subsequently de novo organogenesis is triggered in the neighboring cells. |
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Keywords: | caulogenesis histology morphogenesis protrusions thidiazuron |
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