The History and Advances of Reversible Terminators Used in New Generations of Sequencing Technology |
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| Authors: | Fei Chen Mengxing Dong Meng Ge Lingxiang Zhu Lufeng Ren Guocheng Liu Rong Mu |
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| Affiliation: | CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences,Beijing 100101, China |
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| Abstract: | DNA sequencing using reversible terminators, as one sequencing by synthesis strategy, has garnered a great deal of interest due to its popular application in the second-generation high-throughput DNA sequencing technology. In this review, we provided its history of development, classification, and working mechanism of this technology. We also outlined the screening strategies for DNA polymerases to accommodate the reversible terminators as substrates during polymerization; particularly, we introduced the “REAP” method developed by us. At the end of this review, we discussed current limitations of this approach and provided potential solutions to extend its application. |
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| Keywords: | DNA polymerases Sequencing technology Modified nucleotide Primer extension Reversible terminator Sequencing by synthesis |
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