Comparative genomic hybridisation for the analysis of chromosomal imbalances in solid tumours and haematological malignancies |
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Authors: | H Zitzelsberger Lars Lehmann Martin Werner Manfred Bauchinger |
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Institution: | GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Strahlenbiologie, Postfach 1129, D-85758 Oberschlei?heim, Germany Tel. -49-89-31873421; fax -49-89-31872873 e-mail: Zitzelsberger@gsf.de, DE GSF-National Research Centre for Environment and Health, Institute of Radiobiology, Neuherberg, Germany, DE Technical University Munich, Institute of Pathology, Munich, Germany, DE
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Abstract: | Comparative genomic hybridisation (CGH) is based on a two-colour, competitive fluorescence in situ hybridisation of differentially
labelled tumour and reference DNA to normal metaphase chromosomes. This new technology has made a great impact in molecular
tumour pathology due to its possible application to archival specimens and the ability to create copy number karyotypes throughout
the whole genome from very small amounts of DNA. If chromosomal imbalances can be correlated with a etiological and clinical
features of tumours, CGH could be able to provide new prognostic and diagnostic criteria. CGH findings further provide starting
points for the molecular genetic characterisation of altered chromosomal regions harbouring yet unidentified genes involved
in tumorigenesis and tumour progression. An overview of the results of published CGH studies on solid tumours and haematological
malignancies is presented. Methodological limitations of the CGH technology are reported, as well as future developments which
will improve its use in routine analysis.
Accepted: 29 July 1997 |
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