| BST-2抑制HIV-1病毒样颗粒释放的研究 |
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| 引用本文： | 杜应莲,胡斯奇,庞晓静,岑山,金奇,郭斐. BST-2抑制HIV-1病毒样颗粒释放的研究[J]. 病毒学报, 2011, 27(4): 319-325 |
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| 作者姓名： | 杜应莲 胡斯奇 庞晓静 岑山 金奇 郭斐 |
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| 作者单位： | 中国医学科学院,北京协和医学院,病原生物学研究所病毒基因工程国家重点实验室,北京100730;中国医学科学院,北京协和医学院医药生物技术研究所免疫生物室,北京100050 |
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| 基金项目： | 艾滋病和病毒性肝炎等重大传染病防治科技专项(2009ZX10004-303) |
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| 摘 要： | BST-2是最近发现的可以抑制成熟HIV-1(human immunodeficiency virus,HIV)病毒颗粒从哺乳动物细胞表面释放的宿主因子,随之发现其也可以抑制多种包膜病毒的释放。本研究采用密码子优化的表达HIV-1 gag和gag-pol蛋白的质粒所形成的病毒样颗粒作为研究对象,观测BST-2对这两种病毒样颗粒(Virus-like particle,VLP)的释放抑制情况及其作用机制。结果发现,瞬时表达和稳定表达的BST-2均可以显著抑制病毒样颗粒从哺乳动物细胞释放,同时发现这两种病毒样颗粒(gag/gag-pol)的释放都可以被BST-2抑制;而且,HIV-1中Vpu蛋白可以拮抗BST-2抑制HIV病毒样颗粒释放的作用,另外,通过化学试剂和酶学方法处理,确证BST-2可以被包装进病毒样颗粒中。
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| 关 键 词： | BST-2 人类免疫缺陷病毒 细胞限制因子 病毒释放 |
Inhibition of HIV virus-like particles production by BST-2 |
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| Du Ying-Lian,Hu Si-Qi,Pang Xiao-Jing,Cen Shan,Jin Qi,Guo Fei. Inhibition of HIV virus-like particles production by BST-2[J]. Chinese journal of virology, 2011, 27(4): 319-325 |
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| Authors: | Du Ying-Lian Hu Si-Qi Pang Xiao-Jing Cen Shan Jin Qi Guo Fei |
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| Affiliation: | State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China. |
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| Abstract: | Recently, BST-2 has been identified as an effective cellular factor that prevents the release of human immunodeficiency virus type 1 and other enveloped viruses by tethering virus particles to the cell surface. Here, we showed that the production of HIV-1 virus-like particles was markedly inhibited by BST-2. Both the transient and stable expressing of BST-2 had the same function and Vpu rescued the release of HIV-1 VLP in the presence of human BST-2. Consistent with a direct tethering mechanism, we confirmed that proteolysis releases restricted virions and further showed that this removed the ectodomain of BST-2 from the cell surface. |
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