Elimination of Mycoplasma orale from the human hepatoma cell line, PLC/PRF/5 |
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Authors: | K J Fowler R M Brown R G Cotton |
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Affiliation: | Birth Defects Research Institute, Royal Children''s Hospital Research Foundation, Parkville, Victoria 3052, Australia |
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Abstract: | We have tried without success to eliminate M. orale from a human hepatoma line, PLC/PRF/5, using five different methods. We report here the successful elimination of the contamination by a modification of the technique of Marcus et al. [1] using 5-bromodeoxy-uridine (BrdU) instead of 5-bromo-uracil (5-BrUra) and light. We believe that this method may prove useful when rare and valuable cell lines carry the more common mycoplasma contaminant M. orale. |
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