Extensive structural conservation exists among several homologs of two Euglena chloroplast group II introns |
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Authors: | M. D. Thompson L. Zhang L. Hong R. B. Hallick |
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Affiliation: | (1) Department of Biochemistry, University of Arizona, Tucson, AZ 85721, USA Fax: +1-520-621-1697; e-mail: hallick@arizona.edu, US;(2) University of California, Department of Molecular and Cellular Biology, Berkeley, CA 94720, USA, US |
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Abstract: | 82 of the 155 chloroplast introns in Euglena gracilis have been categorized as group II introns. Because they are shorter and more divergent than group II introns from other organisms, the assignment of these Euglena introns to the group II class has been questioned. In the current study, two homologs of E. gracilispetB intron 1 and four homologs of psbC intron 2 have been isolated from related species and characterized. Based on a comparative sequence analysis of intron homologs, the intron core and four of the six helical domains present in the canonical group II intron structural model are conserved in E. gracilispetB intron 1 and psbC intron 2 and all of their homologs. Distal portions of domain I, which are involved in most of the tertiary interactions, are less well conserved than the central core. Received: 27 June 1997 / Accepted: 6 August 1997 |
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Keywords: | Euglena Chloroplast Group II intron Evolution |
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