Chlamydia trachomatis in cell culture |
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Authors: | Teresa Rondon Rota |
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Institution: | (1) Department of Microbiology, Harvard School of Public Health, 665 Huntington Avenue, 02115 Boston, Massachusetts |
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Abstract: | Summary Trachoma organisms of serotype B were grown serially in irradiated cells (McCoy, BHK-21, Microbiological Associates, and BHK-21,
Lister) and tested for infectivity in monolayers of five mammalian cell lines (BHK-21, CHO, HeLa S3, McCoy and OWMK) and two diploid strains (ST/BTL and WI-38). All cell types had low susceptibility to chlamydial infection
but the number of inclusions increased when the inoculum was centrifuged onto the monolayers, or when the cells were irradiated.
Infection was higher in non-irradiated CHO than in irradiated CHO in three out of a total of six experiments. Inclusion number
was increased 300 times in HeLa S3 and up to three times in the other cell types after treatment with diethylaminoethyl-dextran (DEAE-D). Serial passage of
Chlamydia trachomatis serotype B (strain Har-36) in CO60 McCoy and CO60 BHK-21 Lister resulted in partial adaptation of the strain to the host cell. The phenomenon of adaptation of serotype B to
McCoy compensated for the lower susceptibility of this cell revealed when McCoy cells were inoculated with trachoma elementary
bodies grown in BHK-21 Lister or in chick embryo yolk sac. Trachoma organisms of immunotypes A, B and C prepared in yolk sac
produced more inclusion-forming units per ml in CO60 BHK-21 Lister than in CO60 McCoy.
This research was supported by a grant from the National Eye Institute (EI-00812-08), and by the Arabian American Oil Company.
The paper is dedicated to the memory of Francis B. Gordon, who pioneered research methods for the cultivation of trachoma
and inclusion conjunctivitis (TRIC) agents in cell culture. Dr. Gordon patiently studied tables and photographs which accompany
this text when he visited our laboratory on the day prior to his sailing to England on the ill-fated voyage in which he and
Mrs. Gordon perished (October 1973). |
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Keywords: | trachoma cell culture McCoy BHK-21 |
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