Specific high-affinity binding protein for human corticotropin-releasing hormone in normal human plasma |
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| Authors: | D N Orth C D Mount |
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| Institution: | 1. Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232 USA;2. Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, Tennessee 37232 USA;1. OGS (Istituto Nazionale di Oceanografia e di Geofisica Sperimentale), 34010 Sgonico, TS, Italy;2. Department of Earth and Atmospheric Sciences, University of Alberta, 1-26 Earth Sciences Building, Edmonton, Alberta T6G 2E3, Canada;1. Department of Petroleum Engineering, King Fahd University of Petroleum & Minerals, 31261 Dhahran, Saudi Arabia;2. Center for Integrative Petroleum Research, King Fahd University of Petroleum & Minerals, 31261 Dhahran, Saudi Arabia;1. College of Chemistry and Material Science, Hebei Normal University, Shijiazhuang, 050024, PR China, PR China;2. Key Laboratory of Inorganic Nanomaterial of Hebei Province, Shijiazhuang, 050024, PR China, PR China;1. Duke University School of Medicine, Durham, North Carolina;2. Duke-Margolis Center for Health Policy, Duke University, Durham, North Carolina;3. Department of Pediatrics, Children’s Health and Discovery Initiative, Duke University School of Medicine, Durham, North Carolina;4. Sanford School of Public Policy, Duke University, Durham, North Carolina;5. National Clinician Scholars Program, Duke University School of Medicine, Durham, North Carolina;6. Duke Clinical Research Institute, Duke University School of Medicine, Durham, North Carolina |
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| Abstract: | A protein of Mr 25-40 kilodaltons in normal human plasma binds human corticotropin-releasing hormone (hCRH), but not ovine CRH. Binding requires both N- and C-terminal hCRH sequences and has a Kd of 2 X 10(-10) M and a binding site concentration of 1.4 pmoles per ml of plasma. Its binding is not affected by heating to 56 degrees C for 1 h, but is abolished by exposure to 6 M guanidine-HCl, 10 mM dithiothreitol. Binding proceeds rapidly at 37 degrees C and is 75% complete within 4 min. Neither rat nor sheep plasma appears to contain a CRH-binding protein. CRH-binding protein may explain the brief biological action of hCRH as compared to ovine CRH in man and why high concentrations of plasma immunoreactive hCRH in women during third-trimester pregnancy do not cause increased ACTH secretion. |
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