双歧杆菌发酵果蔬汁对小鼠免疫调节机制的研究

目的研究双歧杆菌发酵果蔬汁对小鼠免疫调节作用的机制。方法将小鼠随机分成纯净水对照组、非发酵果蔬汁组和发酵果蔬汁低、中、高三个剂量组,饮水法喂饲小鼠30d,检测小鼠脾细胞的增殖能力及T淋巴细胞亚群,ELISA法测定血清IL-6和IFN-γ水平。结果发酵果蔬汁可增强小鼠脾细胞的增殖能力,T细胞表面标志CD3、CD4、CD8表达增强,促进血清中IL-6和IFN-γ的生成。结论双歧杆菌发酵果蔬汁能提高小鼠的体液、细胞免疫和非特异性免疫功能,并呈剂量效应关系。

Study on immune mechanism of Bifidobacterium fermented mixed fruit and vegetable juice in mice

Objective To explore the immune mechanism of Bifidobacterium fermented mixed fruit and vegetable juice in mice. Method Mice were randomly assigned to 5 groups ： the purified water （control） group, non-fermented mixed fruit and vegetable juices group and 3 fermented mixed fruit and vegetable juices groups of low, medium and high doses. After feeding for 30 days, the spleen cell proliferation and T lymphocyte subsets of mice were evaluated. The levels of IL- 6 and IFN-γ were determined by ELISA assay. Result The proliferation of spleen ceils and the expression of T cell surface markers CD3, CD4 and CD8 in all 3 fermented mixed fruit and vegetable juice groups were enhanced, and the levels of serum IL-6 and IFN-γ were promoted. Conclusion Bifidobacterium fermented mixed fruit and vegetable juice can enhance the humoral, cellular immune and non-specific immune funetion, presenting a dose-effect relationship.