Optimizing some factors affecting protease production under solid state fermentation

Production of extracellular alkaline protease by a locally isolated fungal species, Rhizopus oryzae, under solid state fermentation was optimized. The maximum enzyme activity under the optimum conditions of temperature (32?°C), relative humidity (90%–95%), spore count (～2?×?10⁵/g wheat bran), moisture content of solid substrate (140%) adjusted suitably with salt solution (M-9) of pH?5.5 was 341 unit/g wheat bran.