A bicistronic baculovirus vector for transient and stable protein expression in mammalian cells |
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Authors: | Lackner Andreas Genta Kathrin Koppensteiner Herwig Herbacek Irene Holzmann Klaus Spiegl-Kreinecker Sabine Berger Walter Grusch Michael |
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Affiliation: | aDepartment of Medicine I, Institute of Cancer Research, Medical University of Vienna, A-1090 Vienna, Austria;bDepartment of Neurosurgery, Wagner Jauregg Hospital, A-4020 Linz, Austria |
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Abstract: | Baculoviruses are widely used for protein production in insect cells, and their potential for gene transfer to mammalian cells is increasingly being recognized. Here we describe a baculovirus vector with a bicistronic mammalian expression cassette and demonstrate its suitability for efficient transient and stable protein expression in human glioblastoma cells. Bicistronic baculovirus vectors are safe, cost efficient, and easy to produce; thus, they represent an excellent gene transfer system for mammalian cells. |
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