多基因系统发育分析竹黄的分类地位

竹黄Shiraia bambusicola是一种重要的药用子囊菌,对其分类归属一直存在不同处理意见,主要原因在于形态分类学难以解释其不同的特征表现.为了对竹黄的分类地位做出更接近自然亲缘关系的解释,本研究以自测的S.bambusicolaS4201菌株基因组为基础,结合Genome Warehouse(GWH)数据库中...

Taxonomic clarification of Shiraia bambusicola based on multigene phylogenetic analysis

Shiraia bambusicola is an important medicinal species of ascomycetes, and there have been disputed on its classification and affiliation mainly due to difficulty in morphological characterization of species. In this study, based on self-testing genome of S. bambusicola S4201, combined with the genomic data of S. bambusicola GZAAS2.1243 and Shiraia sp. slf14 catalogued in the database of Genome Warehouse (GWH) and GenBank respectively, the classification status of S. bambusicola was reexplained more closely to the nature affinity. Four gene sequences, nrSSU, nrLSU, tef-1α and rbp2, were selected for the phylogenetic analysis at order level, in comparison with 74 species of ascomycetes catalogued in the Assembling The Fungal Tree of Life (AFTOL); three gene sequences, ITS, nrLSU and tub2, were selected for the phylogenetic analysis at family level, in comparison with 30 species of Pleosporales accessioned in GenBank database. Maximum likelihood (ML) and Bayesian inference (BI) analyses were used to construct phylogenetic trees, respectively. The results support the establishment of independent Shiraiaceae, and Shiraiaceae should be included in the order Pleosporales. There may exist hidden species in the genus Shiraia.