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耐热黑曲霉3.316菌株全基因组测序及分析
引用本文:高亚男,朱凤妹,李军.耐热黑曲霉3.316菌株全基因组测序及分析[J].菌物学报,2021,40(7):1737-1750.
作者姓名:高亚男  朱凤妹  李军
作者单位:河北科技师范学院 河北 秦皇岛 066600
基金项目:国家自然科学基金(31570374);国家自然科学基金(31470542);河北省自然科学基金(C2015407059)
摘    要:黑曲霉3.316是一株耐热型丝状真菌,最高生长温度达47℃,在工业发酵中有着巨大的应用潜力。为了更加充分地在工业发酵中利用其耐热特性,需要对菌株信息进行全面了解。通过PacBio Sequel测序平台的CLR测序方式对黑曲霉3.316菌株进行全基因组测序。结果表明,基因组最后得到15个contigs,总长度为34 956 132bp,GC含量为49.21%,预测到10 032个编码基因,在GO、KOG、KEGG数据库分别有6 901、2 118和9 494个基因得到注释。通过分析比较得到黑曲霉耐热性与抗氧化相关基因超氧化物歧化酶(SOD)、过氧化氢酶(CAT)有密切关系,这为后续研究黑曲霉3.316耐热特性提供可靠信息,同时为应对工业发酵中的高能耗、高生产成本以及高温环境的现状提供优良菌株。

关 键 词:黑曲霉  全基因组测序  基因功能注释  
收稿时间:2020-12-11

Whole-genome sequencing and analysis of a thermotolerant strain Aspergillus niger 3.316
Authors:GAO Ya-Nan  ZHU Feng-Mei  LI Jun
Institution:Hebei Normal University of Science & Technology, Qinhuangdao, Hebei 066600, China
Abstract: Aspergillus niger 3.316 is a heat-resistant filamentous fungus strain with a maximum growth temperature of 47°C, having an application potential for fermentation industry. In order to make full use of its functionality in industrial fermentation, a comprehensive understanding of the strain information is required. The whole genome of Aspergillus niger 3.316 strain was sequenced through CLR sequencing method of the PacBio Sequel sequencing platform. The results showed that the genome finally obtained 15 contigs with a total length of 34 956 132bp and GC content of 49.21%, and 10 032 coding genes were predicted. There are 6 901, 2 118 and 9 494 genes annotated in the GO, KOG, and KEGG databases, respectively. Through analysis and comparison, it is found that the heat tolerance of Aspergillus niger is closely related to the antioxidant-related genes superoxide dismutase (SOD) and catalase (CAT). This finding provides reliable information for the follow-up study on the heat resistance characteristics of Aspergillus niger 3.316, and solving the high energy consumption, high production costs and high temperature environment affecting industrial fermentation.
Keywords:Aspergillus niger  whole genome sequencing  gene function annotation  
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