三种分离自冬虫夏草真菌及培养条件

对采自青海玉树、青海果洛和云南迪庆的冬虫夏草鲜品进行虫生真菌的分离、纯化,获得3种生长形态不同的真菌QH 2019、GL 2019和YN 2019。通过形态学及分子鉴定,菌株QH 2019为蝙蝠蛾拟青霉Samsoniella hepiali,菌株GL 2019为粉棒束孢Isaria farinosa,菌株YN 2019为玫烟色棒束孢Isaria fumosorosea。对这3株菌的培养条件初步研究,结果表明：菌株QH 2019在1/4 SDAY培养基上菌丝日生长速率最快,为(1.94±0.55)mm/d,且菌丝致密、粗壮,含水率高达(91.90±1.22)%,虫草素和虫草酸均以PDA培养的含量最高,分别为(0.47±0.022)mg/g和(3.24±0.021)mg/g;菌株GL 2019在PDA培养基上菌丝日生长速率、菌丝含水率、虫草素含量和虫草酸含量都最高,分别为(2.37±0.20)mm/d、(88.34±2.00)%、(0.23±0.013)mg/g和(6.92±0.019)mg/g,但其菌丝在1/4 SDAY培养基上生长最为致密、粗壮;菌株YN 2019在PDA培养基上菌丝日生长速率、虫草素和虫草酸的含量最高,分别为：(2.27±0.27)mm/d、(0.50±0.012)mg/g和(11.32±0.16)mg/g,但其菌丝在1/4 SDAY培养基上含水率最高(95.23±1.65)%,且生长最为致密、粗壮。综合评价表明3株真菌中YN 2019菌丝中虫草素和虫草酸的含量高、生长速率快,有较好的药用研究价值。

Three fungal strains isolated from stroma of Ophiocordyceps sinensis and their culture conditions

Three fungal strains were isolated from fresh stroma of Ophiocordyceps sinensis collected from Yushu, Guoluo of Qinghai and Diqing of Yunnan and they were identified as Samsoniella hepiali (QH 2019), Isaria farinosa (GL 2019) and Isaria fumosorosea (YN 2019) based on phylogenetic evidence. The highest mycelial growth rate of QH 2019 was observed on 1/4 SDAY medium, with quantitative value of (1.94±0.55)mm/d, and the mycelia were dense, with water content of (91.90±1.22)%. The highest content of cordycepin and cordycepic acid reaching (0.47±0.022)mg/g and (3.24±0.021)mg/g respectively was observed in culture on PDA. The mycelial growth rate, mycelial water content, cordycepin and cordycepic acid of GL 2019 were the highest in the culture growing on PDA, reaching (2.37±0.20)mm/d, (88.34±2.00)%, (0.23±0.013)mg/g and (6.92±0.019)mg/g respectively, but the mycelium grew much densely on 1/4 SDAY medium. The mycelial growth rate, cordycepin and cordycepic acid of YN 2019 were the highest in the culture growing on PDA, reaching (2.27±0.27)mm/d, (0.50±0.012)mg/g and (11.32±0.16)mg/g respectively, but the mycelium growing on 1/4 SDAY medium was much dense, with the highest mycelial water content of (95.23±1.65)%. The results suggested that Isaria fumosorosea YN 2019 had potential medicinal values.