| 结球甘蓝自交不亲和系组织快繁体系研究 |
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| 引用本文： | 张振超,戴忠良,潘跃平,姚悦梅. 结球甘蓝自交不亲和系组织快繁体系研究[J]. 西北植物学报, 2009, 29(10) |
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| 作者姓名： | 张振超 戴忠良 潘跃平 姚悦梅 |
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| 作者单位： | 镇江丘陵地区农业科学研究所,江苏句容,212400 |
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| 基金项目： | 江苏省科技支撑项目，江苏丘陵地区农业科学研究所所基金项目 |
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| 摘 要： | 对结球甘蓝自交不亲和系进行快繁,以探讨不同外植体、激素配比及浓度、定植方式等因素对结球甘蓝不定芽、不定根发生能力等的影响.结果表明:当激素浓度组合为5 mg/L 6-BA+0.25 mg/L NAA时不定芽分化率最高,腋芽和下胚轴分别为87.5%和85%;当组合为2 mg/L 6-BA+0.01 mg/L NAA和3 mg/L 6-BA+0.1 mg/L NAA时最易诱导产生不定芽,增殖系数达到4以上;适合生根培养基为2/3 MS+0.1 mg/L IBA+0.1 mg/L NAA,平均每株生根数是7～8条,生根率80.3%.移栽到穴盘的试管苗成活率高达100%,生长健壮;直接栽在大田的成活率为84.7%,长势较差.起始培养时腋芽比种子增殖速度快,后期增殖及生长二者无显著差异.
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| 关 键 词： | 甘蓝 自交不亲和系 组织培养 |
Tissue Rapid Reproduction Technology of Self-incompatible Line in Headed Cabbage |
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| ZHANG Zhen-chao,DAI Zhong-liang,PAN Yue-ping,YAO Yue-mei. Tissue Rapid Reproduction Technology of Self-incompatible Line in Headed Cabbage[J]. Acta Botanica Boreali-Occidentalia Sinica, 2009, 29(10) |
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| Authors: | ZHANG Zhen-chao DAI Zhong-liang PAN Yue-ping YAO Yue-mei |
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| Affiliation: | ZHANG Zhen-chao,DAI Zhong-liang,PAN Yue-ping,YAO Yue-mei(Zhenjiang Agricultural Research Institute,Jurong,Jiangsu 212400,China) |
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| Abstract: | The factors of explants,hormone combination and concentration and plant mode were investigated on tissue rapid reproduction technology of self-incompatible line in headed cabbage.The results indicated that the best medium to induce callus was MS+6-BA 5 mg/L+NAA 0.25 mg/L and regeneration rate were 87.5% and 85%,respectively.The media of 2 mg/L 6-BA+0.01 mg/L NAA and 3 mg/L 6-BA+0.1 mg/L NAA were the best for inducing adventitious buds.The plantlet in vitro multiplication coefficience was more than 4.It is e... |
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| Keywords: | headed cabbage self-incompatible line tissue culture |
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