The CD4+CD28null and the regulatory CD4+CD25High T-cell phenotypes in patients with ulcerative colitis during active and quiescent disease, and following colectomy |
| |
Authors: | Yokoyama Yoko Fukunaga Ken Ikeuchi Hiroki Kamikozuru Koji Hida Nobuyuki Ohda Yoshio Iimuro Masaki Yoshida Koji Kikuyama Risa Kato Kyouichi Nagase Kazuko Nakamura Shirou Miwa Hiroto Matsumoto Takayuki |
| |
Institution: | aDivision of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine, Hyogo, Japan;bDivision of Upper Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine, Hyogo, Japan;cDepartment of Surgery, Hyogo College of Medicine, Hyogo, Japan |
| |
Abstract: | The CD4+CD25High T-cell phenotype has an essential immunoregulatory role, while the CD4+CD28null T-cell reflects immune pathology. We investigated the profiles of the CD4+CD25High and the CD4+CD28null T-cell phenotypes in patients with ulcerative colitis (UC) during active and quiescent phases as well as following colectomy. Fifty-nine UC patients, 34 active (UCa) and 25 quiescent (UCq) together with 19 healthy controls (HC) were included. Ten of 34 UCa patients underwent colectomy due to unremitting UC (UCo). Immunohistochemical phenotypic of the peripheral blood lymphocytes bearing CD4, CD25 or CD28 was done for analyzes by a multiparameter fluorescence activated cell sorting technique. The expression of the CD4+CD25High phenotype was higher in UCq (P < 0.01) or UCo (P < 0.01) group vs UCa group. Further, the expression of the CD4+CD28null phenotype in UCa or UCo group was higher than in the HC group (P < 0.05). However, the expression of the CD4+CD28null phenotype up to 12 months after colectomy was not significantly different from the levels in the same patients during acute phase. Our impression is that a high CD4+CD25High T-cell reflects alleviation of inflammation, while the expression of the CD4+CD28null T-cell phenotype is an etiologic feature in UC patients, and is maintained after removing the affected colon. |
| |
Keywords: | Ulcerative colitis Regulatory T-cells CD4+CD28&minus T-cells Immunomodulation Colectomy |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|