Ethylene and polyamine production patterns during in vitro shoot organogenesis of two passion fruit species as affected by polyamines and their inhibitor |
| |
Authors: | Leonardo L C Dias Claudete Santa-Catarina Dimas M Ribeiro Raimundo S Barros Eny I S Floh Wagner C Otoni |
| |
Institution: | 1.Plant Cell Biology Laboratory (BIOCEL), Institute of Biological Sciences, Botany Department,University of S?o Paulo (USP),S?o Paulo,Brazil;2.Cell Biology and Tissue Culture Laboratory, Biosciences and Biotechnology Center,North Fluminense Darcy Ribeiro State University (UENF),Campos dos Goytacazes,Brazil;3.Plant Growth and Development Laboratory, Plant Biology Department,Federal University of Vi?osa (UFV),Vi?osa,Brazil;4.Plant Tissue Culture Laboratory, Plant Biology Department/BIOAGRO,Federal University of Vi?osa,Vi?osa,Brazil |
| |
Abstract: | Levels of ethylene and polyamines (PAs) were measured during organogenesis of hypocotyl explants of two species of passion
fruit (Passiflora cincinnata Masters and Passiflora edulis Sims f. flavicarpa Degener ‘FB-100’) to better understand the relationships of these regulators and their influence on cell differentiation
and morphogenesis. Moreover, histological investigation of shoot ontogenesis was conducted to characterize the different events
involved in cell redifferentiation and regulation of PA and ethylene levels. A delay was observed in morphogenic responses
of P. edulis f. flavicarpa as compared to P. cincinnata, and these changes coincided with production of elevated levels of polyamine and ethylene levels. During differentiation,
cells showed high rates of expansion and elongation, and high ethylene levels were associated with high PA levels, suggesting
that the two biosynthesis pathways were highly regulated. Moreover, their interaction might be an important factor for determining
cell differentiation. The addition of PAs to the culture medium did not promote organogenesis; however, the incorporation
of the PA inhibitor methylglyoxal bisguanylhydrazone in the culture medium reduced shoot bud differentiation, suggesting the
need to maintaining a minimum level of PAs for morphogenic events to take place. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|