Restriction fragment length polymorphism species-specific patterns in the identification of white truffles |
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Authors: | Luana Bertini Lucia Potenza Alessandra Zambonelli Antonella Amicucci Vilberto Stocchi |
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Affiliation: | Istituto di Chimica Biologica Giorgio Fornaini, Universitàdegli Studi di Urbino, Via Saffi 2, 61029 Urbino (PS), Italy;Dipartimento di Protezione e Valorizzazione Agroalimentare, Via Filippo Re, 8-40126 Universitàdi Bologna, Bologna, Italy |
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Abstract: | A molecular method for the identification of ectomycorrhizae belonging to five species of white truffle is described. The polymerase chain reaction (PCR) and universal primers were used to amplify internal transcribed spacers and 5.8S rDNA, target sequences present in a high number of copies. The amplified products were digested with restriction enzymes in order to detect interspecific polymorphisms. Species-specific restriction fragment length polymorphism patterns were determined for all five species. The use of PCR in conjunction with restriction enzymes provides a sensitive and efficient tool for use in distinguishing ectomycorrhizal species and monitoring inoculated seedlings or field mycorrhizal populations. |
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Keywords: | Ectomycorrhizal fungi Restriction fragment length polymorphism Internal transcribed spacer White truffle ectomycorrhizal identification |
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