花生ARAhPR10基因启动子序列的克隆及分析

PR10(pathogenesis-related class10protein)类蛋白与植物的抵御外来病害及系统获得性抗性(SAR)有着紧密联系,本文采用基于PCR的基因组DNA步移法,从抗黄曲霉花生品种粤油20中克隆ARAhPR10(Aspergillus flavus-resistant AhPR10)基因起始密码子ATG上游256bp类似启动子序列,并对其进行植物顺式作用元件数据库PLACE预测分析。结果表明,该类似启动子序列含有4处TATA box和2处CAAT box保守的启动子结构元件,还有6处W-box、1处BIHD1和3处GT-1motif抗逆应答元件,其中W-box常见于PR蛋白的启动子区内参与病程应答。我们初步认为本研究克隆的序列可能是ARAhPR10基因的启动子。

Cloning and Analysis of Promoter-like Sequence of ARAhPR10 Gene from Peanut (Arachis hypogaea L.)

PR10 (pathogenesis-related class10 protein) is considered as a kind of proteins that close associated with the defense of plant diseases and system acquired resistance (SAR).The 256 bp promoter-like sequence of 5’ upstream of initial codon of ARAhPR10 gene from Aspergillus flavus-resistant Arachis hypogaea L.cv.Yueyou20 was cloned by using PCR-based genomic DNA walking approach.The prediction analysis of this promoter-like sequence was carried out based on plant cis-acting element database,PLACE.The results showed that the promoter-like sequence contained conservative structural elements of four TATA boxs and two CAAT boxes,and the promoter response element motifs of six W-boxs,one site of BIHD1 and three sites of GT-1,where W-box usually involved in the PR protein response of promoter region of PR proteins.Our preliminary results indicated that the cloned sequences of this study might be the promoter of ARAhPR10 gene.