在板栗疫病菌线粒体中检出低毒病毒编码蛋白p29

p29蛋白是低毒病毒基因组编码的一个木瓜蛋白酶样蛋白。前人研究发现,在宿主板栗疫病菌(Cryphonectria parasitica)体内表达p29,会引起真菌毒力降低,色素产生减少,丧失产生无性孢子的能力。除已知p29与源于高尔基体的膜结构共分离之外,p29在细胞内的其它分布形式未明。本研究在成功制备p29特异抗体和高效分离板栗疫病菌线粒体的基础上,尝试用p29抗体检测线粒体中是否存在p29蛋白。Western印迹结果表明,受CHV1-EP713感染的EP713菌株线粒体中存在与p29抗体特异作用的病毒蛋白。本研究结果暗示,低毒病毒蛋白p29可能参与调控宿主线粒体功能。

Detection of Hypovirus Protein p29 from Mitochondria of the Chestnut Blight Fungus

p29 is a papain-like protease encoded by hypovirus CHV1-EP713.Previous studies have revealed that expression of p29 in its host,the chestnut blight fungus (Cryphonectria parasitica),results in reduced virulence and suppression of pigment production and asexual sporulation.Except for co-separation with membrane structures originated from the Golgi apparatus,little was known about the subcellular localization of p29 in the cell.With the success in generating antibody specific to p29 and isolation of high quality mitochondria,p29 was detected from the mitochondria of hypovirus-infected C.parasitica by Western Blot analysis.This finding implies that p29 may be involved in perturbation of mitochondrial function of the host fungus.