The effect of sugars on niger embryogenesis and plant regeneration in anther culture |
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Authors: | B P Hema H N Murthy |
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Institution: | (1) Department of Botany, Karnatak University, Dharwad, 580003, India |
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Abstract: | The influence of different sugars (sucrose, maltose, glucose and fructose, 0.05–0.5 M) on embryogenesis and plant regeneration
from cultured anthers of niger Guizotia abyssinica (L. f.) Cass.] have been studied. Among the different sugars tested, 0.2 M sucrose was the best for embryo induction and
plant regeneration. Maximum of 57 embryos per 60 anthers were induced on embryo induction medium Gamborg’s B5 medium supplemented
with 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 2 μM kinetin (KIN)] containing 0.2 M sucrose. Embryo differentiation was
achieved on B5 medium supplemented with 0.5 μM benzyladenine (BA) and 0.09 M sucrose. Embryo maturation was on B5 medium containing
10 μM abscisic acid (ABA) and 0.09 M sucrose. Embryo germination was achieved on B5 medium with 0.09 M sucrose. Embryos that
were developed on B5 medium supplemented with 0.2 M sucrose showed highest frequency (68 %) of plant regeneration. |
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Keywords: | fructose glucose growth regulators Guizotia abyssinica haploids maltose sucrose |
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