Analysis of the phytochrome gene family in <Emphasis Type="Italic">Ceratodon purpureus</Emphasis> by gene targeting reveals the primary phytochrome responsible for photo- and polarotropism |
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Authors: | Franz Mittmann Sven Dienstbach Andrea Weisert Christoph Forreiter |
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Institution: | (1) Department of Plant Physiology, Justus Liebig University, Senckenbergstrasse 3, 35390 Giessen, Germany;(2) Present address: Department of Genetics, Justus Liebig University, Heinrich-Buff Ring 58-62, 35392 Giessen, Germany |
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Abstract: | Using gene targeting by homologous recombination in Ceratodon purpureus, we were able to knock out four phytochrome photoreceptor genes independently and to analyze their function with respect
to red light dependent phototropism, polarotropism, and chlorophyll content. The strongest phenotype was found in knock-out
lines of a newly described phytochrome gene termed CpPHY4 lacking photo- and polarotropic responses at moderate fluence rates. Eliminating the atypical phytochrome gene CpPHY1, which is the only known phytochrome-like gene containing a putative C-terminal tyrosine kinase-like domain, affects red
light-induced chlorophyll accumulation. This result was surprising, since no light dependent function was ever allocated to
this unusual gene.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Accession number for CpPHY4: EU122393. |
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Keywords: | Ceratodon Gene targeting Knock-out Phytochrome |
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