Somatic embryogenesis and plantlet regeneration in mango (Mangifera indica L.) |
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Authors: | Lilian F Pateña Luzminda R Carlos-Refuerzo Ramon C Barba |
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Institution: | 1. Institute of Plant Breeding, College of Agriculture, University of the Philippines Los Ba?os, College, 4031, Laguna, Philippines
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Abstract: | Summary The ‘Carabao’ or ‘Manila Super’ mango (Mangifera indica L.), a virtually neglected fruit before the advent of KNO3 flower induction in the early 1970s, is now the third leading Philippine export fruit after banana and pineapple. To apply
biotechnology for improvement, a reliable embryogenesis and regeneration protocol is required. We have developed a protocol
for somatic embryogenesis and plantlet regeneration in mango: eight strains of ‘Carabao’ and two unidentified varieties, PHL
12384 and PHL 12378. Over 40 batches of nucellar explants from immature fruis (0.75–5.0 cm long) were cultured in vitro from April 1999 to April 2000. Two media were used, MMSE. Mango Medium for Somatic Embryo Induction, Proliferation and Germination
and MMPR, Mango Medium for Plantlet Regeneration. These are now routinely used. The protocol is reproducible in 14 other varieties
of mango. Shifting the base medium from Gamborg's B5 medium to our own formulation. BP medium (Barba and Pate?a's formulation)
effectively controlled browning. Browning has limited the successful in vitro culture of many woody species including the mango.
Crop Science Society of the Philippines (CSSP) 2001 Best Paper Award, Asian Agriculture Congress, Westin Philippine Plaza,
Manila, Philippines, April 24–27, 2001 and Philippine Fruit Association 2000 Best Poster Award, 8th National Symposium. PCARRD,
Los Ba?os, Laguna, Philippines, November 14–16, 2000. |
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Keywords: | ‘ Carabao’ or ‘ Manila Super’ mango BP medium nucellus browning |
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