Evaluation of a digoxigenin-labelled probe for detection of Aeromonas spp. |
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| Authors: | R Khushiramani SK Girisha I Karunasagar I Karunasagar |
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| Institution: | Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore, India |
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| Abstract: | An outer membrane protein-based Digoxigenin (DIG)-labelled DNA probe was developed for the specific detection of Aeromonas sp. from food/environmental/clinical samples. Dot blot reaction answered for all the Aeromonas isolates and was negative for Escherichia coli , Pseudomonas sp., Klebsiella sp., Vibrio parahaemolyticus , V. harveyi , V. alginolyticus , V. vulnificus . Edwardsiella tarda and Staphylococcus sp. As this protein is highly conserved in various Aeromonas species, the probe has the potential for use as a rapid and reliable diagnostic tool. |
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| Keywords: | Aeromonas detection digoxigenin gram-negative bacterium probe |
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