Contrasting roles of oxidized lipids in modulating membrane microdomains |
| |
Authors: | Tayana Mazin Tsubone Helena Couto Junqueira Maurício S Baptista Rosangela Itri |
| |
Institution: | 1. Department of Applied Physics, Institute of Physics, University of São Paulo, SP, Brazil;2. Department of Biochemistry, Institute of Chemistry, University of São Paulo, SP, Brazil |
| |
Abstract: | Lipid rafts display a lateral heterogeneity forming membrane microdomains that hold a fundamental role on biological membranes and are indispensable to physiological functions of cells. Oxidative stress in cellular environments may cause lipid oxidation, changing membrane composition and organization, thus implying in effects in cell signaling and even loss of homeostasis. The individual contribution of oxidized lipid species to the formation or disruption of lipid rafts in membranes still remains unknown. Here, we investigate the role of different structures of oxidized phospholipids on rafts microdomains by carefully controlling the membrane composition. Our experimental approach based on fluorescence microscopy of giant unilamellar vesicles (GUV) enables the direct visualization of the impact of hydroperoxidized POPC lipid (referred to as POPCOOH) and shortened chain lipid PazePC (1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine) on phase separation. We found that the molecular structure of oxidized lipid is of paramount importance on lipid mixing and/or demixing. The hydrophobic mismatch promoted by POPCOOH coupled to its cylindrical molecular shape favor microdomains formation. In contrast, the conical shape of PazePC causes disarrangement of lipid 2D organized platforms. Our findings contribute to better unraveling how oxidized phospholipids can trigger formation or disruption of lipid rafts. As a consequence, phospholipid oxidation may indirectly affect association or dissociation of key biomolecules in the rafts thus altering cell signaling and homeostasis. |
| |
Keywords: | Corresponding author at: Rua do Matão 1371 - Butantã São Paulo SP Brazil Oxidized lipids Membrane domains Lipid rafts GUV |
本文献已被 ScienceDirect 等数据库收录! |
|