The relationship between the presence of antibodies and direct detection of Toxoplasma gondii in slaughtered calves and cattle in four European countries |
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| Authors: | M Opsteegh F Spano D Aubert A Balea A Burrells S Cherchi JBWJ Cornelissen C Dam-Deisz J Guitian A Györke EA Innes F Katzer G Limon A Possenti E Pozio G Schares I Villena HJ Wisselink JWB van der Giessen |
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| Institution: | 1. National Institute for Public Health and the Environment, Centre for Infectious Disease Control, P.O. Box 1, 3720 BA Bilthoven, The Netherlands;2. Department of Infectious Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy;3. National Reference Centre for Toxoplasmosis, Laboratory of Parasitology, EA 7510, University of Reims Champagne-Ardenne and CHU Maison Blanche, Reims, France;4. Parasitology and Parasitic Diseases Department, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 3-5 M?n??tur Street, 400372, Romania;5. Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, United Kingdom;6. Wageningen Bioveterinary Research, P.O. Box 65, 8200 AB Lelystad, The Netherlands;7. The Royal Veterinary College, Veterinary Epidemiology, Economics and Public Health Group, Hawkshead Lane, North Mymms, AL9 7TA Hatfield, United Kingdom;8. Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Epidemiology, Südufer 10, 17493 Greifswald, Insel Riems, Germany |
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| Abstract: | In cattle, antibodies to Toxoplasma gondii infection are frequently detected, but evidence for the presence of T. gondii tissue cysts in cattle is limited. To study the concordance between the presence of anti-T. gondii IgG and viable tissue cysts of T. gondii in cattle, serum, liver and diaphragm samples of 167 veal calves and 235 adult cattle were collected in Italy, the Netherlands, Romania and the United Kingdom. Serum samples were tested for anti-T. gondii IgG by the modified agglutination test and p30 immunoblot. Samples from liver were analyzed by mouse bioassay and PCR after trypsin digestion. In addition, all diaphragms of cattle that had tested T. gondii-positive (either in bioassay, by PCR on trypsin-digested liver or serologically by MAT) and a selection of diaphragms from cattle that had tested negative were analyzed by magnetic capture quantitative PCR (MC-PCR). Overall, 13 animals were considered positive by a direct detection method: seven out of 151 (4.6%) by MC-PCR and six out of 385 (1.6%) by bioassay, indicating the presence of viable parasites. As cattle that tested positive in the bioassay tested negative by MC-PCR and vice-versa, these results demonstrate a lack of concordance between the presence of viable parasites in liver and the detection of T. gondii DNA in diaphragm. In addition, the probability to detect T. gondii parasites or DNA in seropositive and seronegative cattle was comparable, demonstrating that serological testing by MAT or p30 immunoblot does not provide information about the presence of T. gondii parasites or DNA in cattle and therefore is not a reliable indicator of the risk for consumers. |
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| Keywords: | Corresponding author Cattle Serology Mouse bioassay PCR Detection |
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