Aluminium-induced morphogenic and biochemical variations ofBacopa Monniera |
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Authors: | G Ali P S Srivastava M Iqbal |
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Institution: | (1) Department of Botany, Hamdard University, 110062 New Delhi, India |
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Abstract: | Shoots and roots ofBacopa monniera (L.) Wettst. have been regenerated from nodal segments on MS medium containing combinations of NAA and BAP. The cultures
showed 100% regeneration on MS (sucrose 2%) medium added with NAA (0.2 mg L-1), BAP (0.5 mg L-1) and glutamine (50 mg L-1). Supplemented with aluminium chloride (up to 400 μM), this medium could ensure successful survival of regenerants. AH the
regenerants, maintained on AlCl3-supplemented medium for the last three years, failed to grow when transferred to AlCl3-free media. Aluminium stress also induced synthesis of proline and proteins. The rate of photosynthesis decreased at increased
aluminium concentrations. |
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Keywords: | Bacopa monniera aluminium morphogenesis proline regenerants |
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