Embryogenic cell suspensions from the male flower of Musa AAA cv. Grand nain |
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Authors: | F X Côte R Domergue S Monmarson J Schwendiman C Teisson J V Escalant |
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Institution: | F. X. Côte (corresponding author, e-mail ), R. Domergue, S. Monmarson, J. Schwendiman and C Teisson, CIRAD-FLHOR, Laboratoire B10TROP, B.P. 5035, Av. du val de Montferrand, F-34032 Montpellier Cedex, France;J. V. Escalant, CATIE (Centro Agronomico Tropical de Investigation y Ensenanza), Unidad de Biotecnologia, 7170 Turrialba, Costa Rica. |
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Abstract: | There are very few reports on the establishment of long-term embryogenic cell cultures of banana, especially of triploid cultivars of commercial interest. Embryogenic cell suspensions were prepared using the cultivar Grand nain, the most widely grown dessert banana in the world. After culture for 5 or 6 months of immature male flowerbuds adjacent to the floral apex, yellow, compact calluses and white, friable embryogenic tissues were induced. Suspension cultures were initiated from embryogenic tissues placed in liquid medium. The packed cell volume (PCV) of the suspensions increased 2- to 5- fold with each monthly culture cycle. Plating of the embryogenic suspensions resulted in approximately 370×103 embryos per ml of PCV. Depending on the size of embryos, 3 to 20% germination was observed. A histological survey of cell suspensions and embryo development was carried out. Cellular aggregates with cells displaying typical embryogenic features were formed. Most of the somatic embryos were probably of unicellular origin. |
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Keywords: | Banana embryogenic cell suspension micropropaganon Musa somatic embryogenesis |
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