Cartilaginous tissue formation from bone marrow cells using rotating wall vessel (RWV) bioreactor |
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Authors: | Ohyabu Yoshimi Kida Naoko Kojima Hiroko Taguchi Tetsushi Tanaka Junzo Uemura Toshimasa |
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Institution: | Nanotechnology Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba Central-6, Tsukuba, Ibaraki 305-8566, Japan. |
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Abstract: | This is the first successful report of the rapid regeneration of three-dimensional large and homogeneous cartilaginous tissue from rabbit bone marrow cells without a scaffold using a rotating wall vessel (RWV) bioreactor, which simulates a microgravity environment for cells. Bone marrow cells cultured for 3 weeks in DMEM were resuspended and cultured for 4 weeks in the chondrogenic medium within the vessel. Large cylindrical cartilaginous tissue with dimensions of (1.25 +/- 0.06) x (0.60 +/- 0.08) cm (height x diameter) formed. Their cartilage marker expression was confirmed by mRNA expressions of aggrecan, collagen type I and II, and glycosaminoglycan (GAG)/DNA ratio. Their cartilaginous properties were demonstrated by toluidine blue, safranin-O staining, and polarization. |
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Keywords: | cartilage tissue regeneration bone marrow cell RWV bioreactor |
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