Identification of a Novel Garlic Cellulase Gene |
| |
Authors: | Aeri Kim Ryong Nam Kim Dae-Won Kim Sang-Haeng Choi Aram Kang Seong-Hyeuk Nam Hong-Seog Park |
| |
Institution: | (1) Genome Research Center and Industrial biotechnology and bioenergy research center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-306, South Korea;(2) University of Science and Technology (UST), Daejeon, 303-333, South Korea |
| |
Abstract: | Genes encoding cellulase enzymes have been investigated in various plants due to the importance of cellulase enzymes in industrial
applications, especially in the conversion of biomass into biofuels. Although several cellulase genes have been cloned and
characterized, little is known about cellulase genes from garlic or enzyme activities of their gene products. In this study,
a cellulase gene from garlic was cloned and characterized in gene and protein levels for the first time. The DNA sequence
of the garlic cellulase gene showed 81% identity with the sequence of the endo-beta-1,4-glucanase of Pisum sativum. The open reading frame of this gene is 1,506 bp, which corresponds to 501 deduced amino acids. We identified the novel ORF
region, which was translated into a 55.2 kDa protein using the protein expression vector, pET28a, in Escherichia coli and we confirmed that this protein has cellulase activity in vitro. Our study demonstrates that garlic is very useful, not
only for the culinary and pharmaceutical industries, but also as an excellent natural source of various kinds of important
genes and enzymes. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|