Purification and characterization of an endochitinase produced by Colletotrichum gloeosporioides |
| |
| Authors: | Souza R F Gomes R C Coelho R R R Alviano C S Soares R M A |
| |
| Institution: | Departamento de Microbiologia Geral, Instituto de Microbiologia Prof Paulo de Góes (IMPPG), Centro de Ciências da Saúde, Universidade do Brasil, Ilha do Fund?o, CEP 21941-590, Rio de Janeiro, RJ, Brazil. |
| |
| Abstract: | The phytopathogenic fungus Colletotrichum gloeosporioides was analyzed for chitinase activity, the best production occurring at the fourth day. A 43 kDa endochitinase with specific activity of 413 U microg(-1) protein was purified corresponding to a 75% yield. The optima of temperature and pH for the enzyme were 50 degrees C and pH 7.0, respectively. The enzyme showed a high stability at 50 degrees C and pH 7.0. Values of pH from 5.0 up to 7.0 gave, at least, 50% of maximum activity, suggesting a biotechnological application. Further studies are in progress to determine the possible use of this endochitinase in biological control. |
| |
| Keywords: | Phytopathogenic fungus Endochitinase Purification Characterization Colletotrichum gloeosporioides |
| 本文献已被 PubMed 等数据库收录！ |
|
