Multipotency and growth characteristic of periosteum-derived progenitor cells for chondrogenic,osteogenic, and adipogenic differentiation |
| |
| Authors: | Yong-Soo Choi Sang-Eun Noh Sang-Min Lim Chang-Woo Lee Chul-Soo Kim Moon-Whan Im Moon-Hee Lee Dong-Il Kim |
| |
| Affiliation: | (1) Department of Biological Engineering, Inha University, Incheon, 402-751, Korea;(2) Boryung Pharmaceutical Co. Ltd, Ansan, GyeongGi, 425-120, Korea;(3) Good Shepherd Hospital, Yeoksam-dong, Seoul, 135-717, Korea;(4) Division of Hematology-Oncology, Inha University Hospital, Incheon, 400-711, Korea;(5) Department of Obstetrics & Gynecology, Inha University Hospital, Incheon, 400-711, Korea |
| |
| Abstract: | The mesengenic multipotency of cryopreserved periosteum-derived progenitor cells (PDPCs) for chondrogenesis, osteogenesis and adipogenesis was investigated. Differentiation was verified using RT-PCR and histological analysis. For characterization, FACS analysis was performed with specific surface markers of mesenchymal stem cells (MSCs). Among PDPCs, unsorted periosteum-derived cells (PDCs) and dermal fibroblasts, the most distinct characteristics were found to be CD9, CD105, and CD166. In addition, these markers in PDPCs were continuously maintained until passage 15. We developed a rapid method for the isolation of PDPCs that can differentiate into mesodermal lineages and provide enough cells in a short period of time for allogeneic cell therapy. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
| |
| Keywords: | Cell therapy Mesenchymal stem cells Multipotency Periosteum-derived progenitor cells |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
