Fibrinogenolytic toxin from Indian monocled cobra (Naja kaouthia) venom |
| |
Authors: | C Chandra Sekhar Dibakar Chakrabarty |
| |
Institution: | Department of Biological Sciences, Birla Institute of Technology and Science-Pilani, KK Birla Goa Campus, Zuarinagar, Goa 403726, India. |
| |
Abstract: | A fibrinogenolytic toxin of molecular weight 6.5 kDa has been purified from the venom of Indian monocled cobra (Naja kaouthia) by repeated cation exchange chromatography on CM-sephadex C-50. The purified toxin did not show any phospholipase activity but was mildly hemolytic on human erythrocytes. This toxin, called Lahirin, cleaved fibrinogen in a dose- and time-dependent manner. The digestion process apparently started with the A alpha chain, and gradually other lower-molecular-weight chains were also cleaved to low-molecular-weight peptides. The fibrinolytic activity was completely lost after treatment with ethylene di-amine tetra acetic acid (EDTA). However, exposure to 100 degree C for 1 min or pre-treatment with phenyl methyl sulfonyl fluoride (PMSF) did not affect the fibrinolytic activity. Cleavage of di-sulphide bonds by beta-mercaptoethanol or unfolding the protein with 4 M urea caused complete loss of activity of pure Lahirin. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|