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Hexokinase microheterogeneity in rabbit red blood cells and its behaviour during reticulocytes maturation
Authors:Vilberto Stocchi  Mauro Magnani  Giovanni Piccoli  Giorgio Fornaini
Institution:(1) Istituto di Chimica Biologica, Universitá degli Studi, Via Saffi, 2, 61029 Urbino, Italy
Abstract:Hexokinase in rabbit reticulocytes is present in two molecular forms (hexokinase Ia and Ib) separable by ion-exchange chromatography on DE-52 columns. By the use of ion-exchange HPLC we have been able to show that the isozymic form we previously called hexokinase la can be resolved into two peaks of activity one of which is (Ia) soluble, the other (Ia*) particulate. Hexokinase Ia* can be solubilized by detergents like saponine and Triton X-100 and disappears during ‘in vivo’ reticulocytes maturation. This new hexokinase micro-heterogeneity is not caused by different oxidized forms of the enzyme nor influenced by the presence of proteolytic inhibitors during lysate preparation.
Keywords:hexokinase heterogeneity  reticulocyte maturation  (rabbit)
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