纳米二氧化铈对神经细胞PC12及SH-SY5Y活力的影响

目的:探究纳米二氧化铈(CeO₂)对神经细胞PC12与SH-SY5Y活力的影响。方法:合成纳米CeO₂材料,并对其结构进行表征,性能进行评估。用不同终浓度(1、2.5、5、10、25、50、75、100、150 μg/ml)的纳米CeO₂分别处理PC12细胞与SH-SY5Y细胞24 h,使用MTT法检测其细胞活力。然后使用活性氧清除剂NAC与纳米CeO₂共孵育处理PC12细胞与SH-SY5Y细胞,并用DCFH-DA探针染色,在荧光倒置显微镜下观察各组细胞的数量及其荧光强度。对实验数据采用单因素方差(one-way ANOVA)分析。结果:纳米CeO₂处理后,PC12细胞(P＜0.01)与SH-SY5Y细胞(P＜0.01)的活力都明显下降,与对照组差异明显。DCFH-DA探针染色后,发现纳米CeO₂的浓度越高,荧光强度越强,表明有活性氧(ROS)的产生。活性氧清除剂NAC与纳米CeO₂(100 μg/ml)共同处理PC12后,荧光强度明显减弱。与25 μg/ml (P＜0.01)、50 μg/ml(P＜0.01)、75 μg/ml(P＜0.01)、100 μg/ml(P＜0.01)纳米CeO₂处理组相比,共同处理组细胞活力明显增加。结论:纳米CeO₂对神经细胞PC12与SH-SY5Y的活力有明显的抑制作用,其机制可能与ROS有关。

Effects of CeO 2 nanoparticles on the viabilities of neural PC12 and SH-SY5Y cells

Objective:To investigate the effects of cerium oxide(CeO 2)nanoparticles on the viabilities of nerve cells PC12 and SH-SY5Y.Methods:CeO 2 nanoparticles were synthesized,structures were characterized and properties were evaluated.PC12 cells and SH-SY5Y cells were treated with CeO 2 nanoparticles at different concentrations(1,2.5,5,10,25,50,75,100,150μg/ml)for 24 h and the cell viability was measured by MTT assay.Then PC12 cells and SH-SY5Y cells were co-treated with CeO 2 and active oxygen scavenger NAC and the cells were stained with DCFH-DA probe for ROS.The number of cells and the fluorescence intensity were observed under a fluorescent inverted microscope.Differences were assessed by one-way ANOVA.Results:After treatment with CeO 2 nanoparticles,the viabilities of both PC12 cells(P<0.01)and SH-SY5Y cells(P<0.01)were decreased comparing with the control group.After staining with DCFH-DA probe,the fluorescence intensity of the nerve cells was enhanced depending on the concentration of CeO 2 nanoparticles suggesting that CeO 2 induced the generation of reactive oxygen species(ROS).The fluorescence intensity of PC12 cells was decreased after CeO 2 nanoparticles(100μg/ml)co-treatment with active oxygen scavenger NAC.Compared with CeO 2 nanoparticles alone at 25μg/ml(P<0.01),50μg/ml(P<0.01),75μg/ml(P<0.01),100μg/ml(P<0.01),the cell viability was significantly increased after co-treatment with NAC.Conclusion:CeO 2 nanoparticles has a negative effect on the viabilities of nerve cells PC12 and SH-SY5Y,and the effect might be depend on ROS.