Cajaflavanone and cajanone released from Cajanus cajan (L. Millsp.) roots induce nod genes of Bradyrhizobium sp. |
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Authors: | Jagroop S. Dahiya |
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Affiliation: | (1) Department of Plant Science, University of Alberta, T6G 2P5 Edmonton, Alberta, Canada |
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Abstract: | A broad-host-range plasmid (pEA2-21) containing a Bradyrhizobium sp (F-4) nod DABC-lacZ translation fusion was constructed and used to monitor nod gene expression in response to pigeonpea root exudate. Two nod-inducing compounds were isolated and identified. Spectral analysis using ultraviolet absorption, infrared spectra, proton nuclear magnetic resonance, and mass spectrometry showed that the two inducers were 5,4-dihydroxy-6-(3-methyl-2-butenyl)-2, 2-dimethyl pyrano-[5, 6:7, 8]-flavanone (cajaflavanone) and 2,4,5-trihydroxy-5-isopentenyl-6, 7-dimethylchromene iso-flavanone (cajanone). When pEA2-21 was introduced into Rhizobium trifolii and R. meliloti cajanone and cajaflavanone did not induce nod gene indicating that specificity of induction appears to be influenced by the host-strain genome. |
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Keywords: | Bradyrhizobium sp. cajanone cajaflavonone /content/v3r11261787w4630/xxlarge946.gif" alt=" beta" align=" MIDDLE" BORDER=" 0" >-galactosidase activity nod-inducers pigeonpea |
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