| PCR及其衍生技术在基因突变检测中的应用 |
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| 引用本文： | 颜志强,杨胜利,龚毅. PCR及其衍生技术在基因突变检测中的应用[J]. 遗传, 2003, 25(2): 198-200 |
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| 作者姓名： | 颜志强 杨胜利 龚毅 |
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| 作者单位： | 中国科学院上海生命科学研究院生物工程研究中心，上海市漕宝路500号 200233Shanghai Research Center of Biotechnology,Institute of Biological Sciences,CAS,Shanghai 200233,China |
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| 基金项目： | 上海市科委发展基金资助项目(00JC14007) |
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| 摘 要： | 许多人类遗传性疾病及某些抗艾滋病药物的抗性乃至细菌对某些抗生素的抗药性通常源于基因突变。本文对近年来在基因突变检测中应用日益广泛的各种PCR 衍生技术作一综述；重点介绍了错配PCR技术，以及我们实验室近期报道的一种快速检测喹诺酮类药物耐药大肠杆菌的错配PCR方案。Abstract:Many inherited diseases and drug resistance have been attributed to mutations in corresponding genes.In this paper,several techniques based on PCR used in diagnosis were concluded.The development and research progress of Mismatch PCR were discussed in details.Some information about an assay that we developed for detection of antimicrobial resistance to quinolones was also described.
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| 关 键 词： | 遗传性疾病 抗药性 错配PCR Key words inherited diseases 基因突变 |
| 文章编号： | 0253-9772(2003)02-0198-03 |
| 修稿时间： | 2002-03-29 |
Use of PCR Related Methods in Detection of Gene Mutation |
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| Zhi-Qiang Yan,Sheng-Li Yang,Yi Gong. Use of PCR Related Methods in Detection of Gene Mutation[J]. Hereditas, 2003, 25(2): 198-200 |
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| Authors: | Zhi-Qiang Yan Sheng-Li Yang Yi Gong |
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| Affiliation: | Shanghai Research Center of Biotechnology, Institute of Biological Sciences, CAS, Shanghai 200233, China. zhiqiangyan@hotmail.com |
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| Abstract: | Many inherited diseases and drug resistance have been attributed to mutations in corresponding genes. In this paper, several techniques based on PCR used in diagnosis were concluded. The development and research progress of Mismatch PCR were discussed in details. Some information about an assay that we developed for detection of antimicrobial resistance to quinolones was also described. |
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| Keywords: | inherited diseases drug resistance gene mutation mismatch PCR |
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