| 毛喉鞘蕊花毛状根培养及二萜化合物的形成 |
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| 引用本文： | 周立刚 胡虹. 毛喉鞘蕊花毛状根培养及二萜化合物的形成[J]. 云南植物研究, 1996, 18(4): 445-450 |
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| 作者姓名： | 周立刚 胡虹 |
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| 作者单位： | [1]华中理工大学药物研究所 [2]中国科学院昆明植物研究所 |
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| 摘 要： | 发根农杆菌ｐＲｉ１５８３４菌株感染毛喉鞘蕊花的根、茎、叶及小苗，均诱导出毛状根。通过纸电泳，在毛状根中检测到甘露碱。该毛状根不仅具有典型的形态特征，并能合成二萜化合物，对其中３个主要成分ｆｏｒｓｋｏｌｉｎ，１，９－ｄｉｄｅｏｘｙｆｏｒｓｋｏｌｉｎ和ｃｏｌｅｏｌ分别进行了定量测定。ＭＳ培养基有利于ｆｏｒｓｋｏｌｉｎ和ｃｏｌｅｏｌ的合成，而Ｂ５培养基促进１，９－ｄｉｄｅｏｘｙｆｏｒｓｋｏｌｉｎ的合成。
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| 关 键 词： | 唇形科 毛喉鞘蕊花 二萜化合物 毛状根 |
FORMATION OF LABDANE DITERPENOIDS BY HAIRY ROOT CULTURES OF COLEUS FORSKOHLII |
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| ZHOU Li-Gang ,HU Hong ,YANG Chong-Ren ,WANG Jun-Jian. FORMATION OF LABDANE DITERPENOIDS BY HAIRY ROOT CULTURES OF COLEUS FORSKOHLII[J]. Acta Botanica Yunnanica, 1996, 18(4): 445-450 |
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| Authors: | ZHOU Li-Gang HU Hong YANG Chong-Ren WANG Jun-Jian |
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| Affiliation: | ZHOU Li-Gang 2,HU Hong 1,YANG Chong-Ren 1 ,WANG Jun-Jian 2 |
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| Abstract: | Hairy root cultures of Coleus forskohlii Briq. were established from intact plant or explants of roots,stems and leaves by infecting with Agrobacterium rhizogenes strain pRi 15834.Transformation was confirmed by paper electrophoresis with mannopine detection. These cultures displayed the typical characteristics of hairy roots.All examined roots formed labdane diterpenoids.Three mainidentified diterpenoids were forskolin, 1,9-dideoxyforskolin and coleol which were measured quantitatively.MS medium favours forskolin and coleol formation, and B 5 medium enhances 1,9-dideoxyforskolin formation. |
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| Keywords: | Coleus forskohlii Briq. Labdane diterpenoids Hairy roots Agrobacterium rhizogenes HPTLC |
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