Influence of Gibberellic Acid on Growth and Endogenous Auxin Level in Epicotyl and Hypocotyl Tissues of Normal and Dwarf Bean Plants |
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Authors: | KRISHAN KUMAR JINDAL TORSTEN HEMBERG |
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Institution: | Botanical Institute, University of Stockholm, Lilla Frescati, S-104 05 Stockholm, Sweden |
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Abstract: | Application of gibberellic acid to the apex of dwarf bean plants (cv. Alabaster) stimulated the elongation growth of epicotyl and hypocotyl but showed no significant effect on elongation growth in a normal cultivar (‘Blue Lake’). Gibberellin-treatment of dwarf plants was characterized by about twofold increase in the level of endogenous auxin. Maximum increase in IAA level was observed after 48 h of GA treatment. There was less increase in IAA content in normal bean plants. — Gibberellin treatment to excised epicotyl and hypocotyl sections of either dwarf or normal cultivar showed no effect on elongation growth. However, a considerable increase in the auxin level was observed in the sections of the dwarf cultivar. The maximum effect occurred with only 1 h incubation in basal medium containing gibberellin. — The indolo-α-pyrone spectro-fluoremetric method for IAA determination was used. |
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